Is it necessary to wear three pairs of gloves when compounding sterile preparations in a containment isolator?

The correct answer is that only two pairs of gloves are required when compounding sterile preparations in a containment isolator. The guidelines set forth in USP 800 indicate that personnel must wear gloves when handling hazardous drugs (HDs), and the use of two pairs of gloves is considered adequate for protecting both the worker and the environment.

Wearing two pairs of gloves allows for an extra layer of protection: the outer glove can provide a barrier against contamination, while the inner glove is in direct contact with the hazardous material. If the outer glove becomes compromised, the inner glove still provides a measure of protection.

Though the use of three pairs of gloves may be mentioned in certain contexts or specific institutional policies, it is not a universal requirement. Additionally, the focus is on minimizing the potential for exposure while maintaining a practical balance in protective measures. This demonstrates the importance of following established guidelines to ensure safety and compliance in handling hazardous materials.